目的 探索麦冬药材有效物质的积累规律,为麦冬生产和质量控制提供理论支持。方法 采集不同发育阶段麦冬块根,采用紫外分光光度法对总多糖、总黄酮、总皂苷含量进行测定,采用HPLC对麦冬甲基黄烷酮A、麦冬皂苷C、麦冬皂苷 D、麦冬皂苷D′、去乙酰基ophiopojaponin A和3-O-α-L-鼠李糖-(1→2)-β-葡萄糖麦冬苷元含量进行测定。结果 麦冬块根形成过程中总皂苷、总黄酮和总多糖的含量和块根生长时间呈正相关,随着块根生长时间的延长,整体呈上升趋势,但在不同的生长期,积累速率不一致,至3月底达到最大值,之后均出现小幅回落;6个单一化学组分积累趋势和大类成分基本一致。同一采集时间,多效唑组样品积累量整体高于无多效唑组样品。结论 本实验发现,麦冬化学成分变化趋势和块根增大趋势基本一致,其中麦冬甲基黄烷酮A和麦冬皂苷D 2个成分最具代表性,可作为评价指标,指导麦冬生产实践和药材质量控制。
Abstract
OBJECTIVE To explore the accumulation rules of the effective constituents in Ophiopogonis Radix to provide theoretical support for its production and quality control. METHODS Ophiopogonis Radix samples in different growth phases were harvested, and the total polysaccharides, total flavones, and total saponins in the sampleswere measured by ultraviolet spectrophotometry. Methylophiopogonanone A, ophiopogonin C, ophiopogonin D, and ophiopogonin D′ were determined by HPLC. RESULTS The contents of total polysaccharides, total flavones, and total saponins in Ophiopogonis Radix were positively correlated with root growth time. The contents of three categories of constituents increased with the prolongation of root growth time, but the accumulative rate was inconsistent in different growth phases, which had a slight decrease after reaching the maximum at the end of March. The accumulation trend of six single components were basically the same to the three categories of constituents. For samples with same harvest time, the content of the main constituents was higher in the samples treated by paclobutrazol than those without paclobutrazol treatment. CONCLUSION The variation trend of the main chemical components in Ophiopogonis Radix is basically the same to that of the root growth. Methylophiopogonanone A and phiopogonin D are the most representative, and they could be taken as evaluation index to guide production and quality control of Ophiopogonis Radix.
关键词
麦冬 /
皂苷 /
黄酮 /
多糖 /
高效液相色谱法
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Key words
Ophiopogonis Radix /
saponin /
flavone /
polysaccharides /
HPLC
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中图分类号：
R282
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参考文献
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脚注
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基金
十二五科技重大专项(2014ZX09304307-002);科技部科技惠民资助项目(2013GS510102);四川省中医管理局资助项目(2014F038);四川省科技厅科技支撑项目(2015SZ0033)
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